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We introduce GROOT, an imitation learning method for learning robust policies with object-centric and 3D priors. GROOT builds policies that generalize beyond their initial training conditions for vision-based manipulation. It constructs object-centric 3D representations that are robust toward background changes and camera views and reason over these representations using a transformer-based policy. Furthermore, we introduce a segmentation correspondence model that allows policies to generalize to new objects at test time. Through comprehensive experiments, we validate the robustness of GROOT policies against perceptual variations in simulated and real-world environments. GROOT's performance excels in generalization over background changes, camera viewpoint shifts, and the presence of new object instances, whereas both state-of-the-art end-to-end learning methods and object proposal-based approaches fall short. We also extensively evaluate GROOT policies on real robots, where we demonstrate the efficacy under very wild changes in setup. 

Manipulating volumetric deformable objects in the real world, like plush toys and pizza dough, brings substantial challenges due to infinite shape variations, non-rigid motions, and partial observability. We introduce ACID, an action-conditional visual dynamics model for volumetric deformable objects based on structured implicit neural representations. ACID integrates two new techniques: implicit representations for action-conditional dynamics and geodesics-based contrastive learning. To represent deformable dynamics from partial RGB-D observations, we learn implicit representations of occupancy and flow-based forward dynamics. To accurately identify state change under large non-rigid deformations, we learn a correspondence embedding field through a novel geodesics-based contrastive loss. To evaluate our approach, we develop a simulation framework for manipulating complex deformable shapes in realistic scenes and a benchmark containing over 17,000 action trajectories with six types of plush toys and 78 variants. Our model achieves the best performance in geometry, correspondence, and dynamics predictions over existing approaches. The ACID dynamics models are successfully employed for goal-conditioned deformable manipulation tasks, resulting in a 30% increase in task success rate over the strongest baseline. Furthermore, we apply the simulation-trained ACID model directly to real-world objects and show success in manipulating them into target configurations. https://b0ku1.github.io/acid/

 

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressingE. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals  <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.